Design and Synthesis of Multi-Functional Superparamagnetic Core-Gold Shell Coated with Chitosan and Folate Nanoparticles for Targeted Antitumor Therapy.
Sharafaldin Al-MusawiSalim AlbukhatyHassan Al-KaragolyFaizah A AlMalkiPublished in: Nanomaterials (Basel, Switzerland) (2020)
A dual-targeting nanomedicine composed of pH-sensitive superparamagnetic iron oxide core-gold shell SPION@Au, chitosan (CS), and folate (FA) was developed as a doxorubicin (DOX) antitumor medication. Microemulsion was used for preparation and cross-linking conjugation. The characteristics of the designed nanocomposite were studied using atomic force microscopy (AFM), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction, UV-visible spectroscopy, Zeta potential and vibrating sample magnetometry (VSM), and Fourier transform infrared spectroscopy. The prepared SPION@Au-CS-DOX-FA nanoparticles (NPs) were spherical with an average diameter of 102.6 ± 7 nm and displayed an elevated drug loading behavior and sustained drug release capacity. The SPION@Au-CS-DOX-FA NPs revealed long term anti-cancer efficacy due to their cytotoxic effect and apoptotic inducing efficiency in SkBr3 cell lines. Additionally, Real-time PCR outcomes significantly showed an increase in BAK and BAX expression and a decrease in BCL-XL and BCL-2. In vivo results revealed that SPION@Au significantly decreased the tumor size in treated mice through magnetization. In conclusion, prepared SPION@Au-CS-DOX-FA could be a beneficial drug formulation for clinical breast cancer treatment.
Keyphrases
- electron microscopy
- drug delivery
- iron oxide
- reduced graphene oxide
- sensitive detection
- cancer therapy
- atomic force microscopy
- drug release
- high speed
- visible light
- single molecule
- real time pcr
- gold nanoparticles
- quantum dots
- poor prognosis
- iron oxide nanoparticles
- healthcare
- adverse drug
- cell death
- high resolution
- computed tomography
- high fat diet induced
- risk assessment
- hyaluronic acid
- human health
- insulin resistance
- induced apoptosis
- metabolic syndrome
- emergency department
- silver nanoparticles
- photodynamic therapy
- climate change
- carbon nanotubes
- bone marrow
- skeletal muscle
- endoplasmic reticulum stress
- optic nerve
- mass spectrometry
- anti inflammatory
- contrast enhanced
- walled carbon nanotubes