High-intensity interval training remodels the proteome and acetylome of human skeletal muscle.
Morten HostrupAnders Krogh LemmingerBen StocksAlba Gonzalez-FranquesaJeppe Kjærgaard LarsenJulia Prats QuesadaMartin ThomassenBrian Tate WeinertJens BangsboAtul Shahaji DeshmukhPublished in: eLife (2022)
Exercise is an effective strategy in the prevention and treatment of metabolic diseases. Alterations in the skeletal muscle proteome, including post-translational modifications, regulate its metabolic adaptations to exercise. Here, we examined the effect of high-intensity interval training (HIIT) on the proteome and acetylome of human skeletal muscle, revealing the response of 3168 proteins and 1263 lysine acetyl-sites on 464 acetylated proteins. We identified global protein adaptations to exercise training involved in metabolism, excitation-contraction coupling, and myofibrillar calcium sensitivity. Furthermore, HIIT increased the acetylation of mitochondrial proteins, particularly those of complex V. We also highlight the regulation of exercise-responsive histone acetyl-sites. These data demonstrate the plasticity of the skeletal muscle proteome and acetylome, providing insight into the regulation of contractile, metabolic and transcriptional processes within skeletal muscle. Herein, we provide a substantial hypothesis-generating resource to stimulate further mechanistic research investigating how exercise improves metabolic health.
Keyphrases
- skeletal muscle
- high intensity
- insulin resistance
- resistance training
- endothelial cells
- physical activity
- healthcare
- public health
- oxidative stress
- dna methylation
- type diabetes
- gene expression
- transcription factor
- amino acid
- metabolic syndrome
- pluripotent stem cells
- drug delivery
- body composition
- social media
- binding protein
- cancer therapy
- smooth muscle
- electronic health record
- virtual reality
- deep learning
- data analysis