Biogenic Polyphosphate Nanoparticles from Synechococcus sp. PCC 7002 Exhibit Intestinal Protective Potential in Human Intestinal Epithelial Cells In Vitro and Murine Small Intestine Ex Vivo.
Guangxin FengYinong FengTengjiao GuoYisheng YangWei GuoMin HuangHaohao WuMingyong ZengPublished in: Journal of agricultural and food chemistry (2018)
Polyphosphates are one of the active compounds from probiotics to maintain gut health. The current research extracted and purified intact biogenic polyphosphate nanoparticles (BPNPs) from Synechococcus sp. PCC 7002 cells. BPNPs were near-spherical anionic particles (56.9 ± 15.1 nm) mainly composed of calcium and magnesium salt of polyphosphate and were colloidally stable at near-neutral and alkaline pH. BPNPs survived gastrointestinal digestion in mice and could be absorbed and transported by polarized Caco-2 cell monolayers. They dose-dependently increased the tightness of intercellular tight junction and the expression of claudin-4, occludin, zonula occludens-1, and heat shock protein 27 in Caco-2 cell monolayers. BPNPs also effectively attenuated H2O2-induced cell death, plasma membrane impairment, and intracellular superoxide production in NCM460 cells. In addition, they conferred resistance to H2O2-induced barrier disruption in freshly excised mouse small intestine. Our results suggest that BPNPs are a promising postbiotic nanomaterial with potential applications in gut health maintenance.
Keyphrases
- cell cycle arrest
- induced apoptosis
- cell death
- heat shock protein
- healthcare
- public health
- high glucose
- endothelial cells
- single cell
- human health
- diabetic rats
- mental health
- cell therapy
- poor prognosis
- endoplasmic reticulum stress
- signaling pathway
- oxidative stress
- photodynamic therapy
- type diabetes
- metabolic syndrome
- drug induced
- risk assessment
- heat shock
- pi k akt
- climate change
- cell proliferation
- hydrogen peroxide
- mesenchymal stem cells
- insulin resistance
- heat stress