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Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii.

Hassan M M AhmedFabienne HeeseErnst A Wimmer
Published in: BMC genetics (2020)
Our results provide evidence that the D. suzukii strain AM derived from the French Alps is more suitable for piggyBac germline transformation than other strains. We demonstrated the feasibility of using φC31-RMCE in the cherry vinegar fly and generated a set of lines that can be used for highly efficient integration of larger constructs. The φC31-based integration will facilitate modification and stabilization of previously generated transgenic lines that carry at least one attP site in the transgene construction. An early embryo-specific and a spermatogenesis-specific driver line were generated for future use of the binary expression system tet-off to engineer tissue- and stage-specific effector gene expression for genetic pest control strategies.
Keyphrases
  • highly efficient
  • gene expression
  • dna methylation
  • escherichia coli
  • genome wide
  • poor prognosis
  • risk assessment
  • climate change
  • copy number
  • dna repair
  • current status
  • dna damage
  • human health
  • high density
  • aedes aegypti