Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells.

Macrophages (M Φ ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M Φ can exist in two extreme phenotypes: pro-inflammatory (M1) M Φ and anti-inflammatory (M2) M Φ . These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M Φ have gained major attention, as they are functionally similar to human monocyte-derived M Φ and are receptive to genome editing. In this study, we polarised iPSC-derived M Φ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised M Φ .