Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts.

Bent Larsen PetersenSvenning Rune MöllerJozef MravecBodil JørgensenMikkel ChristensenYing LiuHans H WandallEric Paul BennettZhang Yang

Published in: BMC biotechnology (2019)

FACS of protoplasts expressing GFP tagged CRISPR/Cas9, delivered through A. tumefaciens leaf infiltration, facilitated clear CRISPR/Cas9 mediated mutation enrichment in selected protoplast populations.

Keyphrases

crispr cas
genome editing
single molecule
single cell
energy transfer
cell therapy
stem cells
protein protein
binding protein
bone marrow
quantum dots
genetic diversity