circHIPK3 regulates proliferation and differentiation of myoblast through the miR-7/TCF12 pathway.
Mengjin GaoXue LiZuojun YangShuo ZhaoXingxing LingJingjing LiKai XingXiaolong QiXiangguo WangLong-Fei XiaoHemin NiYong GuoXihui ShengPublished in: Journal of cellular physiology (2021)
Skeletal muscle development is a complex biological process involving multiple key genes, signaling pathways and noncoding RNAs, including microRNAs and circular RNAs (circRNAs). However, the regulatory relationship among them is so complicated that it has not yet been fully elucidated. In this study, we found that miR-7 inhibited C2C12 cell proliferation and differentiation by targeting transcription factor 12 (TCF12). circHIPK3 acted as a competing endogenous RNA, and its overexpression effectively reversed the regulation of miR-7 on C2C12 cell proliferation and differentiation by increasing TCF12 expression. Taken together, our findings provide evidence that circHIPK3 regulates skeletal muscle development through the miR-7/TCF12 pathway. This study provides a scientific basis for further research on skeletal muscle development at the circRNA level.
Keyphrases
- cell proliferation
- skeletal muscle
- transcription factor
- long non coding rna
- cell cycle
- pi k akt
- insulin resistance
- poor prognosis
- signaling pathway
- long noncoding rna
- type diabetes
- oxidative stress
- gene expression
- adipose tissue
- metabolic syndrome
- epithelial mesenchymal transition
- dna methylation
- nucleic acid
- induced apoptosis