Measurement Biases Distort Cell-Free DNA Fragmentation Profiles and Define the Sensitivity of Metagenomic Cell-Free DNA Sequencing Assays.
Adrienne ChangOmary MzavaJoan S LenzAlexandre P ChengPhilip BurnhamS Timothy MotleyCrissa BennettJohn T ConnellyDarshana M DadhaniaManikkam SuthanthiranJohn R LeeAmy SteadmanIwijn De VlaminckPublished in: Clinical chemistry (2021)
Substantial gains in the sensitivity of microbial and other short fragment recovery can be achieved by easy-to-implement changes in the sample preparation protocol, which highlights the need for standardization in the liquid biopsy field.