Role of genetic variations of DNA damage response pathway genes and heat-shock proteins in increased head and neck cancer risk.
Ishrat MahjabeenSadeeq ShesheTehmina ShakoorMuhammad Zahid HussainMuhammad RizwanAzher MehmoodMuhammad Shahbaz HarisFalak FazalAyesha BurkiMahmood Akhtar KayaniPublished in: Future oncology (London, England) (2022)
Aim: The present study was designed to evaluate the role of DNA damage response pathway genes and heat-shock proteins in head and neck cancer (HNC) risk. Methods: For this purpose, two study cohorts were used. Cohort 1 (blood samples of 250 HNC patients and 250 controls) was used for polymorphism screening of selected genes using tetra-primer amplification refractory mutation system-polymerase chain (Tetra-ARMS PCR). Cohort 2 (200 HNC tumors and adjacent controls) was used for expression analysis, using quantitative PCR. Results: Analysis showed that mutant allele frequency of selected polymorphisms was found associated with increased HNC risk. Expression analysis showed the significant deregulation of selected genes in patients. Conclusion: The present study showed that selected genes ( CHK1, CHK2, HSP70 and HSP90 ) can act as good diagnostic/prognostic markers in HNC.
Keyphrases
- heat shock
- dna damage response
- genome wide
- end stage renal disease
- heat stress
- heat shock protein
- genome wide identification
- ejection fraction
- newly diagnosed
- chronic kidney disease
- dna repair
- prognostic factors
- poor prognosis
- bioinformatics analysis
- peritoneal dialysis
- dna methylation
- high resolution
- dna damage
- copy number
- patient reported outcomes
- transcription factor
- long non coding rna