Functional interrogation and mining of natively paired human VH:VL antibody repertoires.
Bo WangBrandon J DeKoskyMorgan R TimmJiwon LeeErica NormandinJohn MisasiRui KongJonathan R McDanielGeorge DelidakisKendra E LeighThomas NiezoldChang W ChoiElise G VioxAhmed FahadAlberto CagigiAurélie PloquinKwanyee LeungEun Sung YangWing-Pui KongWilliam N VossAaron G SchmidtM Anthony MoodyDavid R AmbrozakAmy R HenryFarida LabouneJulie E LedgerwoodBarney S GrahamMark ConnorsDaniel C DouekNancy J SullivanAndrew D EllingtonJohn R MascolaGeorge GeorgiouPublished in: Nature biotechnology (2018)
We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.