Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade.
Sneha BerryNicolas A GiraldoBenjamin F GreenTricia R CottrellJulie E SteinElizabeth L EngleHaiying XuAleksandra OgurtsovaCharles RobertsDaphne WangPeter NguyenQingfeng ZhuSigfredo Soto-DiazJose LoyolaInbal B SanderPok Fai WongShlomit JesselJoshua DoyleDanielle SignerRichard WiltonJeffrey S RoskesMargaret EminizerSeyoun ParkJoel C SunshineElizabeth D ThompsonAlexander S BarasAngelo M DeMarzoSuzanne L TopalianHarriet KlugerLeslie CopeEvan J LipsonLudmila V DanilovaRobert A AndersDavid L RimmDrew M PardollAlexander S SzalayJanis M TaubePublished in: Science (New York, N.Y.) (2021)
Next-generation tissue-based biomarkers for immunotherapy will likely include the simultaneous analysis of multiple cell types and their spatial interactions, as well as distinct expression patterns of immunoregulatory molecules. Here, we introduce a comprehensive platform for multispectral imaging and mapping of multiple parameters in tumor tissue sections with high-fidelity single-cell resolution. Image analysis and data handling components were drawn from the field of astronomy. Using this "AstroPath" whole-slide platform and only six markers, we identified key features in pretreatment melanoma specimens that predicted response to anti-programmed cell death-1 (PD-1)-based therapy, including CD163+PD-L1- myeloid cells and CD8+FoxP3+PD-1low/mid T cells. These features were combined to stratify long-term survival after anti-PD-1 blockade. This signature was validated in an independent cohort of patients with melanoma from a different institution.
Keyphrases
- single cell
- high throughput
- high resolution
- fluorescence imaging
- rna seq
- poor prognosis
- induced apoptosis
- regulatory t cells
- acute myeloid leukemia
- bone marrow
- dendritic cells
- cell therapy
- cell cycle arrest
- electronic health record
- photodynamic therapy
- skin cancer
- nk cells
- machine learning
- mass spectrometry
- high density
- basal cell carcinoma
- fine needle aspiration
- pi k akt