Login / Signup

In Situ Heterodyne-Detected Second-Harmonic Generation Study of the Influence of Cholesterol on Dye Molecule Adsorption on Lipid Membrane.

Changhui YuPeng ShangYuan GuoZhen Zhang
Published in: The journal of physical chemistry. B (2024)
Cholesterol plays an essential role in regulating the functionality of biomembranes. This study employed in situ second-harmonic generation (SHG) to investigate the adsorption behavior of the dye molecule 4-(4-(diethylamino)styryl)- N -methyl-pyridinium iodide (D289) on a biomimic membrane composed of 1,2-dipalmitoyl- sn -glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (DPPG) and cholesterol. The time-dependent polarization SHG intensity exhibited an initial rapid increase, followed by a subsequent decline. The initial increased SHG intensity is responsible for the electrostatic interaction-driven adsorption of D289 onto the membrane, while the decrease in the SHG signal results from the broadening of the orientation distribution within the membrane. Heterodyne-detected SHG (HD-SHG) measurements demonstrated that the adsorption of dye molecules influenced the phase of the induced electric field. The interfacial potential Φ(0) as a function of time was measured, and we found that even after reaching a stable Stern layer state, the diffusion layer continued to exhibit a dynamic change. This study offers a comprehensive understanding of the influence of cholesterol on adsorption, reorientation dynamics, and dynamic changes in the reorientation of water in the diffusion layer.
Keyphrases
  • aqueous solution
  • risk assessment
  • highly efficient
  • fatty acid
  • climate change
  • diabetic rats
  • human health
  • endothelial cells