A rapid and versatile tool for genomic engineering in Lactococcus lactis.

Tingting GuoYongping XinYi ZhangXinyi GuJian Kong

Published in: Microbial cell factories (2019)

The work provided a rapid, versatile and precise tool for L. lactis genomic engineering by combination of ssDNA recombineering with improved CRISPR/Cas9 counterselection. This tool will simplify the production of isogenic strains for assessment of gene function or construction of biosynthetic host.

Keyphrases

crispr cas
copy number
genome editing
escherichia coli
loop mediated isothermal amplification
dna methylation
transcription factor
quantum dots