Genome Mining Reveals a Multiproduct Sesterterpenoid Biosynthetic Gene Cluster in Aspergillus ustus.

Jingjing Guo You-Sheng CaiFangcai ChengChenjie YangWenqi ZhangWulin YuJingjing YanZixin DengKui Hong

Published in: Organic letters (2021)

Genome mining of Aspergillus ustus 094102 enabled the discovery of a multiproduct bifunctional terpene synthase (BTS), AuAS. Heterologous expression of AuAS led to the discovery of five new sesterterpenes, and coexpression of the upstream CYP450 monooxygenase (AuAP450) generated four new sesterterpene alcohols. Additionally, aspergilol A showed cytotoxic activities against MCF-7, MDA-MB231, and HepG2 cancer cells (IC50 21.20-48.76 μM), and aspergilol B exhibited a cytotoxic effect on MCF-7 cells (IC50 27.41 μM).

Keyphrases

breast cancer cells
genome wide
small molecule
cell cycle arrest
induced apoptosis
high throughput
poor prognosis
cell wall
copy number
endoplasmic reticulum stress
binding protein
oxidative stress
pi k akt
gene expression
cell proliferation
single cell