Correlation between IRC7 gene expression and 4-mercapto-4-methylpentan-2-one production in Saccharomyces cerevisiae strains.
Rosanna TofaloGiorgia PerpetuiniNoemi BattistelliFabrizia TittarelliGiovanna SuzziPublished in: Yeast (Chichester, England) (2020)
Volatile thiols are not present in must but are synthesized and released by wine yeasts during alcoholic fermentation. In this study, autochthonous and commercial Saccharomyces cerevisiae strains were characterized for the expression of the main genes involved in thiols metabolism and their production in wine. New primer sets were developed on the basis of the S288c genome to evaluate the expression of Cys3, Cys4, MET17 and IRC7 genes. Obtained data revealed the occurrence of some thiols, for example, 4-mercapto-4-methylpentan-2-one (4-MMP) and 3-mercaptohexan-1-ol (3-MH) in Pecorino white wine. All genes were upregulated, but only for IRC7 was found a correlation with 4-MMP release: strains with the highest production showed the highest transcription level. IRC7 gene could be proposed as target for the selection of S. cerevisiae strains to increase thiols content in wine.
Keyphrases
- saccharomyces cerevisiae
- escherichia coli
- genome wide
- gene expression
- poor prognosis
- genome wide identification
- dna methylation
- binding protein
- risk assessment
- genome wide analysis
- copy number
- transcription factor
- electronic health record
- single cell
- long non coding rna
- cell migration
- big data
- machine learning
- high resolution
- liquid chromatography
- deep learning
- bioinformatics analysis