Human and murine fibroblast single cell transcriptomics reveals fibroblast clusters are differentially affected by ageing, and serum cholesterol.
Kim van KuijkIan R McCrackenRenée J H A TillieS E J AsselberghsDlzar A KhederS MuitjensHan JinR S TaylorR Wichers SchreurChristoph KuppeRoss DobiePrakash RamachandranMarion J J GijbelsLieve TemmermanPhoebe M KirkwoodJ LuytenYanming LiHeidi NoelsPieter G GoossensJohn R Wilson-KanamoriLeon J SchurgersY H ShenBarend M E MeesErik A L BiessenNeil Cowan HendersonRafael KramannAndrew H BakerJudith C SluimerPublished in: Cardiovascular research (2023)
We provide two adventitial fibroblast-specific markers, PDGFRA and DPEP1, and demonstrate fibroblast heterogeneity in health and cardiovascular disease in humans and mice. Biological relevance is evident from regulation of fibroblast clusters by age and hypercholesterolemia in vivo, associations with human atherosclerotic plaque traits, and enrichment of genes with a GWAS for CVD.
Keyphrases
- single cell
- cardiovascular disease
- endothelial cells
- rna seq
- public health
- induced pluripotent stem cells
- healthcare
- genome wide
- wound healing
- mental health
- type diabetes
- high throughput
- cardiovascular events
- gene expression
- metabolic syndrome
- climate change
- skeletal muscle
- adipose tissue
- social media
- transcription factor