Application of CRISPR-Based Human and Bacterial Ribosomal RNA Depletion for SARS-CoV-2 Shotgun Metagenomic Sequencing.

Stacey CerónNathan C ClemonsBenjamin von BredowShangxin Yang

Published in: American journal of clinical pathology (2022)

The CRISPR-based human and bacterial rRNA depletion enhanced the sensitivity of SARS-CoV-2 shotgun metagenomic sequencing, especially in low viral load samples.

Keyphrases

sars cov
endothelial cells
crispr cas
genome editing
genome wide
induced pluripotent stem cells
single cell
antibiotic resistance genes
microbial community
coronavirus disease