LncRNA MBNL1-AS1 Suppresses Cell Proliferation and Metastasis of Pancreatic Adenocarcinoma through Targeting Carcinogenic miR-301b-3p.
Chouman Sulidankazha AlidakeHai LinTieying HeWei HanQilong ChenPublished in: Genetics research (2023)
Pancreatic adenocarcinoma (PAAD) has been a huge challenge to public health due to its increasing incidence, frequent early metastasis, and poor outcome. The molecular basis of tumorigenesis and metastasis in PAAD is largely unclear. Here, we identified a novel tumor-suppressor long noncoding RNA (lncRNA) MBNL1-AS1, in PAAD and revealed its downstream mechanism. Quantitative real-time PCR (qRT-PCR) data showed that MBNL1-AS1 expression was significantly downregulated in PAAD tissues and cells, which was closely associated with metastasis and poor prognosis. Cell counting kit-8 (CCK-8) assay, transwell assay, and western blot verified that overexpression of MBNL1-AS1 suppressed cell proliferation, migration, and epithelial mesenchymal transformation (EMT) behavior in PAAD cells. By using a dual luciferase reporter gene system, we confirmed that miR-301b-3p was a direct target of MBNL1-AS1. Further mechanismic study revealed that upregulation of miR-301b-3p abolished the inhibitory effect of MBNL1-AS1 overexpression on cell proliferation, tumorigenesis, migration and EMT. Our results demonstrate that MBNL1-AS1 plays a tumor-suppressive role in PAAD mainly by downregulating miR-301b-3p, providing a novel therapeutic target for PAAD.
Keyphrases
- cell proliferation
- poor prognosis
- long noncoding rna
- long non coding rna
- induced apoptosis
- public health
- cell cycle arrest
- cell cycle
- pi k akt
- real time pcr
- single cell
- signaling pathway
- epithelial mesenchymal transition
- high throughput
- stem cells
- bone marrow
- endoplasmic reticulum stress
- transcription factor
- electronic health record
- cell therapy
- risk factors
- high resolution
- crispr cas
- mesenchymal stem cells
- machine learning
- cancer therapy
- copy number
- genome wide
- drug delivery
- deep learning