Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2-1A and GmFAD2-1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean.
Phat T DoCuong X NguyenHien T BuiLy T N TranGary StaceyJason D GillmanZhanyuan J ZhangMinviluz G StaceyPublished in: BMC plant biology (2019)
Overall, our data showed that dual gRNA CRISPR/Cas9 system offers a rapid and highly efficient method to simultaneously edit homeologous soybean genes, which can greatly facilitate breeding and gene discovery in this important crop plant.
Keyphrases
- highly efficient
- crispr cas
- genome editing
- genome wide
- genome wide identification
- genome wide analysis
- bioinformatics analysis
- dna methylation
- small molecule
- transcription factor
- climate change
- electronic health record
- copy number
- high throughput
- gene expression
- big data
- machine learning
- deep learning
- artificial intelligence
- plant growth