Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network.
Ella S KimArjun RajanKathleen ChangSanath GovindarajanClara GulickEva EnglishBianca RodriguezOrion BloomfieldStella NakadaCharlotte BeardSarah O'ConnorSophia MastroianniEmma DowneyMatthew FeigenbaumCaitlin TolentinoAbigail PaceMarina KhanSoyoun MoonJordan DiPrimaAmber SyedFlora LinYasmina AbukhadraIsabella BaconJohn BeckerleSophia ChoNana Esi DonkorLucy GarbergAva HarringtonMai HoangNosa LawaniAyush NooriEuwie ParkElla ParsonsPhilip OravitanMatthew ChenCristian MolinaCaleb RichmondAdith ReddiJason HuangCooper ShugrueRose CovielloSelma UnverMatthew IndelicartoEmir IslamovicRosemary McIlroyAlana YangMahdi HamadElizabeth GriffinZara AhmedAsha AllaPatricia FitzgeraldAudrey ChoiTanya DasYuchen ChengJoshua YuTabor RoderiquesEthan LeeLongchao LiuJaekeb HarperJason WangChris SuhrMax TanJacqueline LuqueA Russell TamEmma ChenMax TriffLyric ZimmermannEric ZhangJackie WoodKaitlin ClarkNat KpodonuAntar DeyAlexander EckerMaximilian ChuangRamón Kodi Suzuki LópezHarry SunZijing WeiHenry StoneChia Yu Joy ChiAiden SilvestriPetra OrloffNeha NedumaranAletheia ZouLeyla ÜnverOscair PageMinseo KimTerence Yan Tao ChanAkili TullochAndrea HernandezAruli PillaiCaitlyn ChenNeil ChowdhuryLina HuangAnish MudideGarrett PaikAlexandra WingateLily QuinnChris ConybereLuca Laiza BaumgardtRollo BuckleyZara KolbergRuth PattisonAshlyn Ahmad ShazliPia GanskeLuca SfragaraAnnina StrubBarney CollierHari TamanaDylan RavindranJames HowdenMadeleine StewartSakura ShimizuJulia BraniffMelanie FongLucy GutmanDanny IrvineSahil MalholtraJillian MedinaJohn ParkAlicia YinHarrison AbromavageBreanna BarrettJacqueline ChenRachelle ChoMac DilatushGabriel GawCaitlin GuJupiter HuangHouston KilbyEthan MarkelKatie McClureWilliam PhillipsBenjamin PolaskiAmelia RoselliSoleil Saint-CyrEllie ShinKylan TatumTai TumpunyawatLucia WetherillSara PtaszynskaMaddie ZeleznikAlexander PesendorferAnna NolanJeffrey TaoDivya SammetaLaney NicholsonGiao Vu DinhMerrin FoltzAn VoMaggie RossAndrew TokarskiSamika HariharanElaine WangMartha BaziukAshley TayYuk Hung Maximus WongJax FloydAileen CuiKieran PierreNikita CoppisettiMatthew KutamDhruv KhurjekarAnthony GadziBen GubbaySophia PedrettiSofiya BelovichTiffany YeungMercy FeyLayla ShafferArthur LiGiancarlo BeritelaKyle HuyghueGreg FosterGarrett Durso-FinleyQuinn ThierfelderHolly KiernanAndrew LenkowskyTesia ThomasNicole ChengOlivia ChaoPia L'Etoile-GogaAlexa KingParis McKinleyNicole ReadDavid MilbergLeila LinMelinda WongIo GilmanSamantha BrownLila ChenJordyn KosaiMark VerbinskyAlice Belshaw-HoodHonon LeeCathy ZhouMaya LoboAsia TseKyle TranKira LewisPratmesh SonawaneJonathan NgoSophia ZuzgaLillian ChowVianne HuynhWenyi YangSamantha LimBrandon StitesShannon ChangRaenalyn Cruz-BallezaMichaela PeltaStella KujawskiChristopher YuanElio Standen-BloomOliver WittKarina AndersAudrey DuaneNancy HuynhBenjamin LesterSamantha Fung-LeeMelanie FungMandy SituPaolo CanigiulaMatijs DijkgraafWilbert RomeroSamantha Karmela BaulaKimberly WongIvana XuBenjamin MartinezReena NuygenLucy NorrisNoah NijensohnNaomi AltmanElise MaajidOlivia BurkhardtJullian ChandaCatherine DoscherAlex GopalAaron GoodJonah GoodNate HerreraLucas LantingSophia LiemAnila MarksEmma McLaughlinAudrey LeeCollin MohrEmma PattonNaima PyaraliClaire OczonDaniel RichardsNathan GoodSpencer GossAdeeb KhanReagan MadoniaVivian MitchellNatasha SunTarik VrankaDiogo GarciaFrida ArroyoEric MoralesSteven CameyGiovanni CanoAngelica BernabeJennifer ArroyoYadira LopezEmily GonzalezBryan ZumbaJosue GarciaEsmeralda VargasAllen TrinidadNoel CandelariaVanessa ValdezFaith CampuzanoEmily PereznegronJenifer MedranoJonathan GutierrezEvelyn GutierrezEricka Taboada AbregoDayanara GutierrezCristian OrtizAngelica BarnesEleanor ArmsLeo MitchellCiara BalanzáJake BradfordHarrison DetroyDevin FergusonEthel GuillermoAnusha ManapragadaDaniella NanulaBrigitte SernaKhushi SinghEmily SramatyBrian WellsMatthew WigginsMelissa DowlingGeraldine SchmadekeSamantha CafferkyStephanie GoodMargaret ReeseMiranda FleigAlex GannettCory CainMelody LeePaul ObertoJennifer RinehartElaine PanSallie Anne MathisJessica JoinerLeslie BarrCory J EvansAlberto Baena-LopezAndrea BeattyJeanette ColletteRobert SmullenJeanne SuttieTownley ChisholmCheryl RotondoGareth LewisVictoria TurnerLloyd StarkElizabeth FoxAnjana AmirapuSangbin ParkNicole LantzAnne E RankinSeung K KimLutz KockelPublished in: G3 (Bethesda, Md.) (2023)
Conditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic and tissue expression studies of 301 novel Stan-X LexA enhancer traps derived from mobilization of the index SX4 line. This includes insertions into distinct loci on the X, II and III chromosomes that were not previously associated with enhancer traps or targeted LexA constructs, an insertion into ptc, and seventeen insertions into natural transposons. A subset of enhancer traps was expressed in CNS neurons known to produce and secrete insulin, an essential regulator of growth, development and metabolism. Fly lines described here were generated and characterized through studies by students and teachers in an international network of genetics classes at public, independent high schools, and universities serving a diversity of students, including those underrepresented in science. Thus, a unique partnership between secondary schools and university-based programs has produced and characterized novel resources in Drosophila, establishing instructional paradigms devoted to unscripted experimental science.