Genome-Wide Survey Indicates Diverse Physiological Roles of Dendrobium officinale Calcium-Dependent Protein Kinase Genes.
Xingyu YangZhiyu ChenXin YinYu-Hua WangYunqiang YangYong-Ping YangPublished in: International journal of molecular sciences (2022)
Calcium-dependent protein kinases (CDPKs) are crucial calcium ions (Ca 2+ ) sensors in plants with important roles in signal transduction, plant growth, development, and stress responses. Here, we identified 24 genes encoding CDPK s in Dendrobium officinale using genome-wide analysis. The phylogenetic analysis revealed that these genes formed four groups, with similar structures in the same group. The gene expression patterns following hormone treatments and yeast two-hybrid of homologous CDPK gene pairs with Rbohs showed differences, indicating functional divergence between homologous genes. In addition, the rapid accumulation of hydrogen peroxide (H 2 O 2 ) and stomatal closure was observed in response to salicylic acid (SA)/jasmonic acid (JA) stress. Our data showed that CDPK9-2 and CDPK20-4 interacted with Rboh D and Rboh H, respectively, and were implicated in the generation of H 2 O 2 and regulation of the stomatal aperture in response to salicylic acid/jasmonic acid treatment. We believe these results can provide a foundation for the functional divergence of homologous genes in D. officinale .
Keyphrases
- genome wide
- genome wide analysis
- dna methylation
- genome wide identification
- hydrogen peroxide
- gene expression
- bioinformatics analysis
- dna repair
- copy number
- dna damage
- protein kinase
- nitric oxide
- plant growth
- mass spectrometry
- cross sectional
- oxidative stress
- quantum dots
- transcription factor
- heat stress
- amino acid
- combination therapy
- stress induced
- data analysis
- smoking cessation
- cell wall