The effect of flow separation and turbulence on the performance of a jet pump in oscillatory flows is investigated. A jet pump is a static device whose shape induces asymmetric hydrodynamic end effects when placed in an oscillatory flow. This will result in a time-averaged pressure drop which can be used to suppress acoustic streaming in closed-loop thermoacoustic devices. An experimental setup is used to measure the time-averaged pressure drop as well as the acoustic power dissipation across two different jet pump geometries in a pure oscillatory flow. The results are compared against published numerical results where flow separation was found to have a negative effect on the jet pump performance in a laminar flow. Using hot-wire anemometry the onset of flow separation is determined experimentally and the applicability of a critical Reynolds number for oscillatory pipe flows is confirmed for jet pump applications. It is found that turbulence can lead to a reduction of flow separation and hence, to an improvement in jet pump performance compared to laminar oscillatory flows.