Organotypic 3D Cell-Architecture Impacts the Expression Pattern of miRNAs-mRNAs Network in Breast Cancer SKBR3 Cells.
María de Los Ángeles Gastélum-LópezMaribel Aguilar-MedinaCristina García MataJorge López-GutiérrezJose Geovanni RomeroMercedes Bermudez CortezMariana Melisa AvendañoCesar López-CamarilloCarlos Perez-PlasenciaAdriana S BeltranRosalio Ramos PayánPublished in: Non-coding RNA (2023)
The organotypic 3D cell-organization architecture of breast cancer SKBR3 cells impacts the expression pattern of the miRNAs-mRNAs network mainly through overexpression of hsa-mir-122-5p, hsa-mir-369-3p, and hsa-mir-10b-5p. All these findings suggest that the interaction between cell-cell and cell-ECM as well as the change in the culture architecture impacts gene expression, and, therefore, support the pertinence of migrating breast cancer research from conventional cultures to 3D models.