Kaistella soli sp. nov., isolated from oil-contaminated experimental soil.

A light yellow-coloured, non-motile, aerobic, Gram-stain-negative, and rod-shaped bacterial strain DKR-2 T was isolated from oil-contaminated experimental soil. The strain was catalase and oxidase positive, and grew at 0-1.5% (w/v) NaCl concentration, at temperature 10-35 °C, and at pH 6.0-9.5. The phylogenetic analysis suggested that the strain DKR-2 T was affiliated to the genus Kaistella, with the closest species being Kaistella haifensis DSM 19056 T (97.6% 16S rRNA gene sequence similarity). The principle fatty acids were iso-C 15:0 , summed feature 9 (iso-C 17:1 ω9c and/or C 16:0 10-methyl), and antiso-C 15:0 . The sole menaquinone was MK-6 and major polar lipid was phosphatidylethanolamin. The DNA G+C content was 39.5%. The dDDH (in silico DNA-DNA hybridization) and ANI (average nucleotide identity) values between strain DKR-2 T and K. haifensis DSM 19056 T were 22.4% and 79.3%, respectively. In addition, both dDDH and ANI values between strain DKR-2 T and other phylogenetically related neighbours were < 25.0% and < 77.0%, respectively. In overall, the polyphasic taxonomic data presented in this study clearly indicated that strain DKR-2 T represents a novel species in the genus Kaistella, for which the name Kaistella soli sp. nov. is proposed. The type strain is DKR-2 T (=KACC 22070 T =NBRC 114725 T ).