Tenogenic induction of human adipose-derived stem cells by soluble tendon extracellular matrix: composition and transcriptomic analyses.
Ying RaoChenxian ZhuHoi Ching SuenShuting HuangJinyue LiaoDai Fei Elmer KerRocky S TuanDan WangPublished in: Stem cell research & therapy (2022)
Our findings showed that urea-extracted tECM contained 20% w/w collagens and is significantly enriched with other non-collagenous tendon ECM components. Compared to Col1 treatment, tECM supplementation enhanced hASC proliferation and tenogenic differentiation as well as induced distinct gene expression profiles. These findings provide insights into the potential mechanism of the pro-tenogenic bioactivity of tECM and support the development of future tECM-based approaches for tendon repair.
Keyphrases
- extracellular matrix
- anterior cruciate ligament reconstruction
- rotator cuff
- endothelial cells
- high glucose
- diabetic rats
- signaling pathway
- induced pluripotent stem cells
- gene expression
- single cell
- anti inflammatory
- combination therapy
- dna methylation
- pluripotent stem cells
- climate change
- risk assessment
- genome wide identification