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PD-L1 quantification across tumor types using the reverse phase protein microarray: implications for precision medicine.

Elisa BaldelliK Alex HodgeGuido BellezzaNeil J ShahGuido GambaraAngelo SidoniMartina MandaranoChamodya RuhunusiriBryant DunetzMaysa Abu-KhalafJulia WulfkuhleRosa I GallagherLance LiottaJohann de BonoNiven MehraRuth RiisnaesAntonella RavaggiFranco OdicinoMaria Isabella SereniMatthew BlackburnAngela ZupaGiuseppina ImprotaPerry DemskoLucio Crino'Vienna LudoviniGiuseppe GiacconeEmanuel F PetricoinMariaelena Pierobon
Published in: Journal for immunotherapy of cancer (2022)
Continuous RPPA-based measurements capture a broad dynamic range of PD-L1 expression in human specimens and heterogeneous concordance levels between antibody clones. This high throughput immunoassay can potentially identify subgroups of tumors in which low expression of PD-L1 equates to lack of response to treatment.
Keyphrases
  • high throughput
  • endothelial cells
  • poor prognosis
  • binding protein
  • induced pluripotent stem cells
  • single cell
  • sensitive detection
  • protein protein
  • small molecule
  • long non coding rna
  • fine needle aspiration