Quantitative mRNA imaging throughout the entire Drosophila brain.
Xi LongJennifer ColonellAllan M WongRobert H SingerTimothée LionnetPublished in: Nature methods (2017)
We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).
Keyphrases
- loop mediated isothermal amplification
- single molecule
- label free
- resting state
- high resolution
- white matter
- functional connectivity
- sensitive detection
- cerebral ischemia
- quantum dots
- growth factor
- multiple sclerosis
- blood brain barrier
- reduced graphene oxide
- highly efficient
- brain injury
- metal organic framework
- electron microscopy