Pseudomonas syringae pv. actinidiae Effector HopAU1 Interacts with Calcium-Sensing Receptor to Activate Plant Immunity.
Jinlong ZhangMingxia ZhouWei LiuJiajun NieLili HuangPublished in: International journal of molecular sciences (2022)
Kiwifruit canker, caused by Pseudomonas syringae pv. actinidiae ( Psa ), is a destructive pathogen that globally threatens the kiwifruit industry. Understanding the molecular mechanism of plant-pathogen interaction can accelerate applying resistance breeding and controlling plant diseases. All known effectors secreted by pathogens play an important role in plant-pathogen interaction. However, the effectors in Psa and their function mechanism remain largely unclear. Here, we successfully identified a T3SS effector HopAU1 which had no virulence contribution to Psa , but could, however, induce cell death and activate a series of immune responses by agroinfiltration in Nicotiana benthamiana , including elevated transcripts of immune-related genes, accumulation of reactive oxygen species (ROS), and callose deposition. We found that HopAU1 interacted with a calcium sensing receptor in N. benthamiana (NbCaS) as well as its close homologue in kiwifruit (AcCaS). More importantly, silencing CaS by RNAi in N. benthamiana greatly attenuated HopAU1-triggered cell death, suggesting CaS is a crucial component for HopAU1 detection. Further researches showed that overexpression of NbCaS in N. benthamiana significantly enhanced plant resistance against Sclerotinia sclerotiorum and Phytophthora capsici , indicating that CaS serves as a promising resistance-related gene for disease resistance breeding. We concluded that HopAU1 is an immune elicitor that targets CaS to trigger plant immunity.
Keyphrases
- cell death
- crispr cas
- prostate cancer
- genome editing
- reactive oxygen species
- immune response
- biofilm formation
- candida albicans
- dendritic cells
- cell wall
- plant growth
- staphylococcus aureus
- escherichia coli
- cell proliferation
- pseudomonas aeruginosa
- type iii
- regulatory t cells
- cell cycle arrest
- multidrug resistant
- gram negative
- genome wide
- binding protein
- label free
- loop mediated isothermal amplification