Intermittent fasting modulates the intestinal microbiota and improves obesity and host energy metabolism.
Xiangwei HuKai XiaMinhui DaiXiaofeng HanPeng YuanJia LiuShiwei LiuFuhuai JiaJiayu ChenFangfang JiangJieyao YuHuanming YangJian WangXun XuXin JinKarsten KristiansenLiang XiaoWei ChenMo HanShenglin DuanPublished in: NPJ biofilms and microbiomes (2023)
Intermittent fasting (IF) is a promising paradigm for weight loss which has been shown to modulate the gut microbiota based on 16S rRNA gene amplicon sequencing. Here, 72 Chinese volunteers with a wide range of body mass index (BMI) participated in a three-week IF program during which an average loss of 3.67 kg body weight accompanied with improved clinical parameters was observed irrespective of initial anthropometric and gut microbiota status. Fecal samples were collected before and after the intervention and subjected to shotgun metagenomic sequencing. De novo assembly yielded 2934 metagenome-assembled genomes (MAGs). Profiling revealed significant enrichment of Parabacteroides distasonis and Bacteroides thetaiotaomicron after the intervention, with inverse correlations between their relative abundances and parameters related to obesity and atherosclerotic cardiovascular diseases (ASCVD). MAGs enriched after the intervention showed high richness and diversity of carbohydrate-active enzymes, with an increased relative abundances of genes related to succinate production and glutamate fermentation.
Keyphrases
- weight loss
- insulin resistance
- body mass index
- weight gain
- body weight
- single cell
- randomized controlled trial
- bariatric surgery
- metabolic syndrome
- cardiovascular disease
- type diabetes
- genome wide
- blood glucose
- roux en y gastric bypass
- high fat diet induced
- high intensity
- adipose tissue
- gastric bypass
- skeletal muscle
- copy number
- body composition
- genome wide identification
- quality improvement
- blood pressure
- glycemic control
- physical activity
- gene expression
- microbial community
- dna methylation
- study protocol
- antibiotic resistance genes
- saccharomyces cerevisiae
- obese patients