LncRNA NEAT1/miR-146a-5p Axis Restores Normal Angiogenesis in Diabetic Foot Ulcers by Targeting mafG.
Tca ArchithaGeorge Raj JuanitaaRamanarayanan VijayalalithaRavichandran JayasuriyaGopinathan AthiraRamachandran BalamuruganKumar GanesanKunka Mohanram RamkumarPublished in: Cells (2024)
Non-healing lesions in diabetic foot ulcers are a significant effect of poor angiogenesis. Epigenetic regulators, mainly lncRNA and miRNA, are recognized for their important roles in disease progression. We deciphered the regulation of lncRNA NEAT1 through the miR-146a-5p/mafG axis in the progression of DFU. A lowered expression of lncRNA NEAT1 was associated with dysregulated angiogenesis through the reduced expression of mafG , SDF-1α , and VEGF in chronic ulcer subjects compared to acute DFU. This was validated by silencing NEAT1 by SiRNA in the endothelial cells which resulted in the transcriptional repression of target genes. Our in silico analysis identified miR-146a-5p as a potential target of lncRNA NEAT1. Further, silencing NEAT1 led to an increase in the levels of miR-146a-5p in chronic DFU subjects. This research presents the role of the lncRNA NEAT1/miR-146a-5p/mafG axis in enhancing angiogenesis in DFU.
Keyphrases
- endothelial cells
- vascular endothelial growth factor
- long non coding rna
- poor prognosis
- long noncoding rna
- wound healing
- gene expression
- high glucose
- transcription factor
- dna methylation
- binding protein
- drug induced
- molecular docking
- oxidative stress
- cancer therapy
- heat stress
- human health
- extracorporeal membrane oxygenation
- genome wide identification