A single factor elicits multilineage reprogramming of astrocytes in the adult mouse striatum.
Yunjia ZhangBoxun LiSergio CananziChuanhui HanLei-Lei WangYuhua ZouYang-Xin FuGary C HonChun-Li ZhangPublished in: Proceedings of the National Academy of Sciences of the United States of America (2022)
SignificanceOutside the neurogenic niches, the adult brain lacks multipotent progenitor cells. In this study, we performed a series of in vivo screens and reveal that a single factor can induce resident brain astrocytes to become induced neural progenitor cells (iNPCs), which then generate neurons, astrocytes, and oligodendrocytes. Such a conclusion is supported by single-cell RNA sequencing and multiple lineage-tracing experiments. Our discovery of iNPCs is fundamentally important for regenerative medicine since neural injuries or degeneration often lead to loss/dysfunction of all three neural lineages. Our findings also provide insights into cell plasticity in the adult mammalian brain, which has largely lost the regenerative capacity.
Keyphrases
- single cell
- high throughput
- rna seq
- resting state
- white matter
- functional connectivity
- stem cells
- cell therapy
- genome wide
- mesenchymal stem cells
- spinal cord injury
- small molecule
- cerebral ischemia
- dna methylation
- oxidative stress
- young adults
- multiple sclerosis
- high glucose
- gene expression
- endothelial cells
- drug induced
- tissue engineering