Measurement of Autophagic Activity in Cancer Cells with Flow Cytometric Analysis Using Cyto-ID Staining.
Merve ŞansaçarEmel Başak Gencer AkçokPublished in: Methods in molecular biology (Clifton, N.J.) (2024)
Autophagy is an evolutionarily conserved process providing the energy that cells need to survive, especially in stress situations, through catabolic processes. Considering the dual role of autophagy in cancer cells depending on the cellular context, it is crucial to comprehend the effect of drug candidates put forward to prevent cancer through the autophagy pathway. The CYTO-ID ® Autophagy Detection Kit allows a rapid, specific and quantitative measurement of autophagic activity at the cellular level using a 488 nm-excitable green fluorescent detection reagent via flow cytometer. In this chapter, we present the CYTO-ID ® Autophagy Detection method with a stepwise protocol to monitor the autophagy flux after the application of any compound to suspension cancer cell lines with flow cytometric analysis.
Keyphrases
- cell death
- endoplasmic reticulum stress
- signaling pathway
- cell cycle arrest
- induced apoptosis
- oxidative stress
- loop mediated isothermal amplification
- papillary thyroid
- label free
- randomized controlled trial
- squamous cell carcinoma
- transcription factor
- young adults
- high resolution
- quantum dots
- mass spectrometry
- data analysis
- childhood cancer