Heterologous expression of fungal L-asparaginase: a systematic review.
Kellen Cruvinel Rodrigues AndradeJoel Antônio Cordeiro de AbreuMarina Guimarães BorgesLetícia Santos AbrunhosaAna Luisa Leôncio RodriguesYris Maria Fonseca-BazzoDamaris SilveiraPaula Monteiro SouzaPérola de Oliveira MagalhãesPublished in: Future microbiology (2023)
Aim: To review the available literature about heterologous expression of fungal L-asparaginase (L-ASNase). Materials & methods: A search was conducted across PubMed, Science Direct, Scopus and Web of Science databases; 4172 citations were identified and seven articles were selected. Results: The results showed that heterologous expression of fungal L-ASNase was performed mostly in bacterial expression systems, except for a study that expressed L-ASNase in a yeast system. Only three publications reported the purification and characterization of the enzyme. Conclusion: The information reported in this systematic review can contribute significantly to the recognition of the importance of biotechnological techniques for L-ASNase production.