Enhanced molecular release from elderly bone samples using collagenase I: insights into fatty acid metabolism alterations.
Amir Mohammad MalvandiEsra HalilajMartina FaraldiLaura MangiaviniSimone CristoniValerio LeoniGiovanni LombardiPublished in: Journal of translational medicine (2024)
This work presents a novel approach for bone sample preparation for HRMS metabolomics. The disruption of bone matrix conformation at the molecular level helps the molecular release into the extracting solvent and, therefore, can lead to higher quality results and trustable biomarker discovery. Our results showed β-oxidation alteration in the aged bone sample. Future work covering more patients is worthy to identify the effective therapeutics to achieve healthy aging.
Keyphrases
- bone mineral density
- bone loss
- soft tissue
- end stage renal disease
- small molecule
- fatty acid
- bone regeneration
- newly diagnosed
- ejection fraction
- postmenopausal women
- chronic kidney disease
- single molecule
- mass spectrometry
- high throughput
- nitric oxide
- high resolution
- prognostic factors
- current status
- patient reported outcomes
- single cell
- patient reported