Transcriptomic analysis of mechanism of melanoma cell death induced by photothermal therapy.
Guorong YanLei ShiFuhe ZhangMin LuoGuolong ZhangPei LiuKaili LiuWei R ChenXiuli L WangPublished in: Journal of biophotonics (2021)
Melanoma is a malignancy with poor prognosis. Its incidence rate has been on the rise and it poses high health and economic challenges to different populations. Photothermal therapy (PTT) served as an effective local therapy in treating various tumors, particularly cutaneous carcinoma like melanoma. To fully understand the mechanisms of tumor cell death induced by PTT, we investigated gene expression and immune cells compositions of B16-F10 tumors after PTT treatment. A total of 256 differentially expressed genes (DEGs) were identified, with 215 being downregulated and 41 upregulated by PTT. Functional annotation showed that most DEGs involved in immune response and inflammatory response. Immune cells compositions inference revealed changes in many immune cells including regulatory T cells, M2 macrophage and B cells after PTT treatment. Our results help delineate the mechanism of cell death at the transcriptional level triggered by non-invasive PTT treatment of melanoma without exogenous light absorbing agents.
Keyphrases
- cell death
- gene expression
- poor prognosis
- regulatory t cells
- immune response
- inflammatory response
- healthcare
- public health
- mental health
- risk factors
- adipose tissue
- dendritic cells
- transcription factor
- bone marrow
- combination therapy
- cell proliferation
- risk assessment
- toll like receptor
- oxidative stress
- social media
- atomic force microscopy
- rna seq
- heat shock protein