Indirect somatic embryogenesis of Piper hispidinervum L. and evaluation of the regenerated plants by flow cytometry.

Paulo Cesar Alves de SousaStênio Steferson Silva E SouzaGabriela Ferreira NogueiraInaê Mariê de Araújo Silva-CardosoJonny Everson Scherwinski-Pereira

Published in: Journal, genetic engineering & biotechnology (2022)

This protocol is the first cited in the literature that demonstrates an efficient micropropagation process by somatic embryogenesis of P. hispidinervum. It can be used either to enable large-scale vegetative production or to subsidize germplasm conservation or genetic engineering of P. hispidinervum.

Keyphrases

flow cytometry
copy number
systematic review
randomized controlled trial
genome wide
genome wide identification
gene expression
dna methylation
essential oil