Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level.
Kaixiang ZhangShangshang QinSixuan WuYan LiangJinghong LiPublished in: Chemical science (2020)
Infectious diseases caused by multidrug resistant (MDR) bacterial pathogens are impending threats to global health. Since delays in identifying drug resistance would significantly increase mortality, fast and accurate antibiotic susceptibility tests (ASTs) are critical for addressing the antibiotic resistance issue. However, the conventional methods for ASTs are always labor-intensive, imprecise, complex and slow (taking 2-3 days). To address these issues, some advanced microfluidic systems have been designed for rapid phenotypic and genotypic analysis of antibiotic resistance. This review highlights the recent development of microfluidics-based ASTs at the single-cell or single-molecule level for guiding antibiotic treatment decisions and predicting therapeutic outcomes.
Keyphrases
- high resolution
- single cell
- single molecule
- multidrug resistant
- global health
- rna seq
- infectious diseases
- high throughput
- gram negative
- drug resistant
- public health
- atomic force microscopy
- loop mediated isothermal amplification
- circulating tumor cells
- living cells
- cardiovascular events
- type diabetes
- klebsiella pneumoniae
- antimicrobial resistance
- coronary artery disease
- fluorescent probe