Harnessing 3D printed highly porous Ti-6Al-4V scaffolds coated with graphene oxide to promote osteogenesis.
Hee Jeong JangMoon Sung KangJinju JangDohyung LimSeong-Won ChoiTae-Gon JungHeoung-Jae ChunBong-Ju KimDong Wook HanPublished in: Biomaterials science (2024)
Bone tissue engineering (BTE) strategies have been developed to address challenges in orthopedic and dental therapy by expediting osseointegration and new bone formation. In this study, we developed irregular porous Ti-6Al-4V scaffolds coated with reduced graphene oxide (rGO), specifically rGO-pTi, and investigated their ability to stimulate osseointegration in vivo . The rGO-pTi scaffolds exhibited unique irregular micropores and high hydrophilicity, facilitating protein adsorption and cell growth. In vitro assays revealed that the rGO-pTi scaffolds increased alkaline phosphatase (ALP) activity, mineralization nodule formation, and osteogenic gene upregulation in MC3T3-E1 preosteoblasts. Moreover, in vivo transplantation of rGO-pTi scaffolds in rabbit calvarial bone defects showed improved bone matrix formation and osseointegration without hemorrhage. These findings highlight the potential of combining rGO with irregular micropores as a promising BTE scaffold for bone regeneration.
Keyphrases
- tissue engineering
- reduced graphene oxide
- bone regeneration
- gold nanoparticles
- bone mineral density
- mesenchymal stem cells
- stem cells
- gene expression
- poor prognosis
- long non coding rna
- single cell
- signaling pathway
- cell proliferation
- cell therapy
- dna methylation
- copy number
- postmenopausal women
- binding protein
- deep learning
- body composition