Portulaca oleracea L. are annual herb, which has various pharmacological effects including hepatoprotective property. However, the effect of Portulaca oleracea L. (POL-1) in mice with carbon tetrachloride (CCl 4 )-induced liver fibrosis and its mechanism of action have not been clarified. POL-1 ameliorated the CCl 4 -induced liver fibrosis in mice, as shown by decreased collagen deposition and the decreased expression of liver fibrosis marker collagen I and α-smooth muscle actin (α-SMA) mRNA. In addition, treatment with POL-1 suppressed the proliferation of activated human hepatic stellate cell line (LX-2). POL-1 inhibited the oxidative stress and inflammation in fibrotic livers of mice. Mechanistically, POL-1 inhibited the CCl 4 -induced expression of toll-like receptor-4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor kappa-B (NF-κBp65) p65, Bcl2-associated X (Bax), transforming growth factor-β1 (TGF-β1) and drosophila mothers against decapentaplegic 2 (Smad2) proteins, upregulated B-cell lymphoma -2 (Bcl-2) proteins in livers of mice. These findings suggested that POL-1 attenuated liver fibrosis.
Keyphrases
- liver fibrosis
- nuclear factor
- toll like receptor
- transforming growth factor
- oxidative stress
- diabetic rats
- epithelial mesenchymal transition
- high fat diet induced
- inflammatory response
- signaling pathway
- immune response
- smooth muscle
- high glucose
- poor prognosis
- drug induced
- endothelial cells
- liver injury
- type diabetes
- binding protein
- bone marrow
- dna damage
- metabolic syndrome
- adipose tissue
- pi k akt
- tissue engineering
- anti inflammatory