In Vitro and In Vivo Effect of Peptides Derived from 14-3-3 Paracoccidioides spp. Protein.
Liliana ScorzoniAna Carolina Alves de Paula E SilvaHaroldo Cesar de OliveiraClaudia Tavares Dos SantosJunya L SingulaniPatricia Akemi AssatoCaroline Maria MarcosLariane Teodoro OliveiraNathália Ferreira FregoneziDiego Conrado Pereira RossiLeandro Buffoni Roque da SilvaCarlos Pelleschi TabordaAna Marisa Fusco-AlmeidaMaria José Soares Mendes-GianniniPublished in: Journal of fungi (Basel, Switzerland) (2021)
None of the peptides were toxic to HaCaT (skin keratinocyte), MRC-5 (lung fibroblast), and A549 (pneumocyte) cell lines, and only P1 exhibited antifungal activity against Paracoccidioides spp. The peptides could induce an immune response in G. mellonella. Moreover, the peptides caused a delay in the death of Paracoccidioides spp. infected larvae. Regarding C. elegans, the three peptides were able to increase the expression of the antimicrobial peptides. These peptides had essential effects on different aspects of Paracoccidioides spp. infection showing potential for a therapeutic vaccine. Future studies using mammalian methods are necessary to validate our findings.