Epidemiological Prevalence of Phenotypical Resistances and Mobilised Colistin Resistance in Avian Commensal and Pathogenic E. coli from Denmark, France, The Netherlands, and the UK.
Andrew MeadCandice Billon-LotzRikke Heidemann OlsenBenjamin M C SwiftPascal RichezRichard StablerLudovic PelligandPublished in: Antibiotics (Basel, Switzerland) (2022)
Colistin has been used for the treatment of non-invasive gastrointestinal infections caused by avian pathogenic E. coli (APEC). The discovery of mobilised colistin resistance (mcr) in E. coli has instigated a One Health approach to minimise colistin use and the spread of resistance. The aim of this study was to compare colistin susceptibility of APECs (collected from Denmark n = 25 and France n = 39) versus commensal E. coli (collected from the Netherlands n = 51 and the UK n = 60), alongside genetic ( mcr -1-5) and phenotypic resistance against six other antimicrobial classes (aminoglycosides, cephalosporins, fluoroquinolones, penicillins, sulphonamides/trimethoprim, tetracyclines). Minimum inhibitory concentration (MIC) values were determined using a broth microdilution method (EUCAST guidelines), and phenotypic resistance was determined using disk diffusion. Colistin MIC values of APEC were significantly lower than those for commensals by 1 dilution ( p < 0.0001, Anderson-Darling test), and differences in distributions were observed between countries. No isolate carried mcr -1-5. Three phenotypically resistant isolates were identified in 2/62 APEC and 1/111 commensal isolates. Gentamicin or gentamicin-ceftriaxone co-resistance was observed in two of these isolates. This study showed a low prevalence of phenotypic colistin resistance, with no apparent difference in colistin resistance between commensal E. coli strains and APEC strains.
Keyphrases
- escherichia coli
- klebsiella pneumoniae
- multidrug resistant
- acinetobacter baumannii
- pseudomonas aeruginosa
- drug resistant
- gram negative
- healthcare
- magnetic resonance imaging
- gene expression
- risk factors
- cross sectional
- magnetic resonance
- cystic fibrosis
- genome wide
- small molecule
- dna methylation
- high resolution
- climate change
- computed tomography
- risk assessment
- ms ms
- simultaneous determination
- combination therapy
- health promotion