Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening.
Enrique SentandreuClaudia Fuente-GarcíaOlga PardoMamen OlivánNúria LeónNoelia AldaiVicent YusàMiguel Ángel SentandreuPublished in: Journal of agricultural and food chemistry (2021)
An understanding of biological mechanisms that could be involved in the stress response of animal cattle prior to slaughter is critical to create effective strategies aiming at the production of high-quality meat. The sarcoplasmic proteome of directly extracted samples from normal and high ultimate pH (pHu) meat groups was studied through a straightforward gel-free strategy supported by liquid chromatography hybrid quadrupole-Orbitrap high-resolution mass spectrometry (LC-HRMS) analysis. A stepped proteomic pipeline combining rapid biomarker hunting supported by qualitative protein Mascot scores followed by targeted label-free peptide quantification revealed 26 descriptors that characterized meat groups assayed. The functional study of the proposed biomarkers suggested their relevant role in metabolic, chaperone/stress-related, muscle contractility/fiber organization, and transport activities. The efficiency, flexibility, rapidity, and easiness of the methodology proposed can positively contribute to the creation of innovative proteomic alternatives addressing meat quality assessment.
Keyphrases
- liquid chromatography
- high resolution mass spectrometry
- mass spectrometry
- tandem mass spectrometry
- ultra high performance liquid chromatography
- label free
- gas chromatography
- simultaneous determination
- high performance liquid chromatography
- solid phase extraction
- systematic review
- small molecule
- high resolution
- amino acid
- clinical trial
- oxidative stress
- ms ms
- hyaluronic acid
- sensitive detection
- heat stress
- drug delivery