RNA-sequencing reveals altered skeletal muscle contraction, E3 ligases, autophagy, apoptosis, and chaperone expression in patients with critical illness myopathy.
Monica Llano-DiezWen FuryHaruka OkamotoYu BaiJesper GromadaLars LarssonPublished in: Skeletal muscle (2019)
RNA-seq analysis revealed that the marked muscle atrophy and weakness observed in ICU patients with CIM were associated with the altered expression of genes involved in muscle contraction, newly identified E3 ligases, autophagy and calpain systems, apoptosis, and chaperone expression. In addition, MYOD1, p38 MAPK, and dexamethasone were identified as potential upstream regulators of skeletal muscle gene expression in ICU patients with CIM. Mechanical loading only marginally affected the skeletal muscle transcriptome profiling of ICU patients diagnosed with CIM.
Keyphrases
- skeletal muscle
- single cell
- rna seq
- poor prognosis
- endoplasmic reticulum stress
- gene expression
- oxidative stress
- cell death
- insulin resistance
- intensive care unit
- end stage renal disease
- mechanical ventilation
- cell cycle arrest
- binding protein
- ejection fraction
- chronic kidney disease
- signaling pathway
- newly diagnosed
- long non coding rna
- heat shock protein
- type diabetes
- acute respiratory distress syndrome
- patient reported outcomes
- heat shock
- extracorporeal membrane oxygenation
- late onset
- endoplasmic reticulum
- duchenne muscular dystrophy
- genome wide analysis