Dissection of the potential anti-diabetes mechanism of salvianolic acid B by metabolite profiling and network pharmacology.
Feng-Xiang ZhangShuang-Shuang CuiYu-Lin-Lan YuanChang LiRui-Man LiPublished in: Rapid communications in mass spectrometry : RCM (2022)
A total of 31 metabolites were characterized in rats after ingestion of Sal B at a dosage of 40 mg/kg, including 1 in plasma, 19 in urine, 31 in feces, 0 in heart, 0 in liver, 0 in spleen, 1 in lung, 1 in kidney and 0 in brain. Among them, 18 metabolites were reported for the first time. Phase I reactions of hydrolysis, hydrogenation, dehydroxylation, hydroxylation, decarboxylation and isomerization, and phase II reactions of methylation were found in Sal B. Notably, decarboxylation and dehydroxylation were revealed in Sal B for the first time. The pharmacology network results showed that Sal B and its metabolites could regulate ALB, PLG, ACE, CASP3, MMP9, MMP2, MTOR, etc. The above targets were involved in insulin signaling pathway, PI3K-Akt signaling pathway, HIF-1 signaling pathway, TNF signaling pathway, etc. CONCLUSIONS: The metabolism feature of Sal B in vivo was systematically revealed, and its anti-diabetes mechanism for further pharmacological validations was predicted based on metabolite profiling and network pharmacology for the first time.
Keyphrases
- signaling pathway
- pi k akt
- type diabetes
- cell proliferation
- phase ii
- ms ms
- single cell
- cell cycle arrest
- epithelial mesenchymal transition
- induced apoptosis
- cardiovascular disease
- glycemic control
- clinical trial
- heart failure
- open label
- randomized controlled trial
- rheumatoid arthritis
- deep learning
- cell death
- cell migration
- metabolic syndrome
- white matter
- angiotensin ii
- gene expression
- adipose tissue
- climate change
- insulin resistance
- atrial fibrillation
- phase iii
- neural network
- double blind