Programmable, Multiplexed DNA Circuits Supporting Clinically Relevant, Electrochemical Antibody Detection.
Sara BracagliaSimona RanalloKevin William PlaxcoFrancesco RicciPublished in: ACS sensors (2021)
Current health emergencies have highlighted the need to have rapid, sensitive, and convenient platforms for the detection of specific antibodies. In response, we report here the design of an electrochemical DNA circuit that responds quantitatively to multiple specific antibodies. The approach employs synthetic antigen-conjugated nucleic acid strands that are rationally designed to induce a strand displacement reaction and release a redox reporter-modified strand upon the recognition of a specific target antibody. The approach is sensitive (low nanomolar detection limit), specific (no signal is observed in the presence of non-targeted antibodies), and selective (the platform can be employed in complex media, including 90% serum). The programmable nature of the strand displacement circuit makes it also versatile, and we demonstrate here the detection of five different antibodies, including three of which are clinically relevant. Using different redox reporters, we also show that the antibody-responsive circuit can be multiplexed and responds to different antibodies in the same solution without crosstalk.
Keyphrases
- loop mediated isothermal amplification
- label free
- nucleic acid
- real time pcr
- gold nanoparticles
- healthcare
- public health
- circulating tumor
- single molecule
- cell free
- cancer therapy
- crispr cas
- mental health
- ionic liquid
- sensitive detection
- high throughput
- high resolution
- photodynamic therapy
- health information
- tandem mass spectrometry