Biotechnological approach for improvement of Crambe species as valuable oilseed plants for industrial purposes.

Boosting technological innovation for a sustainable and circular bioeconomy encompasses the use of renewable materials and development of highly effective biotechnological approaches to improve the quality of oilseed crops and facilitate their industrial deployment. The interest in cultivating Crambe as a potential crop is steadily growing due to its low propensity to crossbreeding with other oilseed crops, valuable seed oil composition and a high yield capacity. The main focus is located on Crambe abyssinica as the most adapted into the agriculture and well-studied Crambe species. At the same time, the Crambe genus is one of the most numerous of the Brassicaceae family featuring several underestimated (orphaned) species with useful traits (abiotic stress tolerance, wide range of practical applications). This review features progress in the biotechnological improvement of well-adapted and wild Crambe species starting with aseptic culture establishment and plant propagation in vitro reinforced with the use of genetic engineering and breeding techniques. The aim of the paper is to highlight and review the existing biotechnological methods of both underestimated and well-adapted Crambe species improvment, including the establishment of aseptic culture, in vitro cultivation, plant regeneration and genetic transformation to modify seed oil content and morphological traits of valuable species.