Integrative Bioinformatics Analysis Reveals That miR-524-5p/MEF2C Regulates Bone Metastasis in Prostate Cancer and Breast Cancer.
QingHua TianYingYing LuBiCong YanChun Gen WuPublished in: Computational and mathematical methods in medicine (2022)
Bone metastases are highly prevalent in patients with advanced prostate cancer and breast cancer and have a serious impact on the survival time and quality of life of these patients. It has been reported that microRNAs (miRNAs) are expressed abnormally in different types of cancer and metastases. However, it remains unknown whether the underlying miRNAs are associated with prostate and breast cancer bone metastasis. Differentially expressed miRNAs (DE-miRNAs) and their potential targets in the metastatic process were identified by bioinformatics analysis. Additionally, qPCR confirmed that the miR-524-5p expression was downregulated in prostate and breast cancer cells. The overexpression of miR-524-5p restrained cell proliferation, invasion, and metastasis in prostate and breast cancer cells. Meanwhile, miR-524-5p could target and inhibit the expression of MEF2C, which was verified by a luciferase assay. In conclusion, our data strongly suggest that downregulation of miR-524-5p appears to be a precocious event in prostate and breast cancer, and the miR-524-5p/MEF2C axis plays a novel role in bone metastases from prostate and breast cancers.
Keyphrases
- prostate cancer
- radical prostatectomy
- cell proliferation
- bioinformatics analysis
- breast cancer cells
- benign prostatic hyperplasia
- poor prognosis
- end stage renal disease
- small cell lung cancer
- bone mineral density
- newly diagnosed
- ejection fraction
- chronic kidney disease
- high throughput
- signaling pathway
- cell cycle
- peritoneal dialysis
- long non coding rna
- binding protein
- machine learning
- soft tissue
- big data
- bone regeneration
- pi k akt
- deep learning
- cell migration