Longitudinal On-Column Thermal Modulation for Comprehensive Two-Dimensional Liquid Chromatography.
Mari E CreeseMathew J CreeseJoe P FoleyHernan J CortesEmily F HilderRobert A ShellieMichael C BreadmorePublished in: Analytical chemistry (2016)
Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography is introduced. Modulation optimization involved a systematic investigation of heat transfer, analyte retention, and migration velocity at a range of temperatures. Longitudinal on-column thermal modulation was realized using a set of alkylphenones and compared to a conventional valve-modulator employing sample loops. The thermal modulator showed a reduced modulation-induced pressure impact than valve modulation, resulting in reduced baseline perturbation by a factor of 6; yielding a 6-14-fold improvement in signal-to-noise. A red wine sample was analyzed to demonstrate the potential of the longitudinal on-column thermal modulator for separation of a complex sample. Discrete peaks in the second dimension using the thermal modulator were 30-55% narrower than with the valve modulator. The results shown herein demonstrate the benefits of an active focusing modulator, such as reduced detection limits and increased total peak capacity.
Keyphrases
- liquid chromatography
- mass spectrometry
- high resolution mass spectrometry
- tandem mass spectrometry
- aortic valve
- mitral valve
- solid phase extraction
- simultaneous determination
- cross sectional
- aortic stenosis
- heart failure
- oxidative stress
- climate change
- risk assessment
- air pollution
- diabetic rats
- drug induced
- endothelial cells
- sensitive detection
- loop mediated isothermal amplification