Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice.
Andras TomeszLaszlo SzaboRichard MolnarArpad DeutschRichard DaragoLászló Bence RaposaNowrasteh GhodratollahTimea VarjasBalazs NemethZsuzsanna OrsosEva PozsgaiJozsef L SzentpeteriFerenc BudanIstvan KissPublished in: Cells (2022)
Specific gene and miRNA expression patterns are potential early biomarkers of harmful environmental carcinogen exposures. The aim of our research was to develop an assay panel by using several miRNAs for the rapid screening of potential carcinogens. The expression changes of miR-124-1, miR-212, miR-132, miR-134, and miR-155 were examined in the spleen, liver, and kidneys of CBA/Ca mice, following the 20 mg/bwkg intraperitoneal 7,12-dimethylbenz(a)anthracene (DMBA) treatment. After 24 h RNA was isolated, the miRNA expressions were analyzed by a real-time polymerase chain reaction and compared to a non-treated control. DMBA induced significant changes in the expression of miR-134, miR-132, and miR-124-1 in all examined organs in female mice. Thus, miR-134, miR-132, and miR-124-1 were found to be suitable biomarkers for the rapid screening of potential chemical carcinogens and presumably to monitor the protective effects of chemopreventive agents.
Keyphrases
- cell proliferation
- long non coding rna
- long noncoding rna
- poor prognosis
- metabolic syndrome
- type diabetes
- adipose tissue
- risk assessment
- high throughput
- human health
- climate change
- insulin resistance
- single cell
- endothelial cells
- diabetic rats
- protein kinase
- high fat diet induced
- combination therapy
- genome wide identification