Investigating the Impact of Vitamin A and Amino Acids on Immune Responses in Celiac Disease Patients.

Amino acids (AAs) and vitamin imbalances are observed in celiac disease (CD). This study evaluated the plasma profile of vitamin A and AAs and the expression level of IL-2, IL-4, IL-10, IL-12 and TGFβ in CD patients. A total of 60 children and adults with CD and 40 healthy controls (HCs) were included. The plasma profile of Vitamin A and AAs and the mRNA expression levels of target genes were assessed. Active adult patients exhibited a decrease in Vitamin A levels ( p = 0.04) and an increase in IL-2 ( p = 0.008) and IL-12 ( p = 0.007) mRNA expression compared to the HCs. The treated adult patients showed elevated Serine ( p = 0.003) and Glycine ( p = 0.04) levels, as well as increased IL-12 ( p < 0.0001) mRNA expression, and a decrease in Tryptophan ( p = 0.04) levels relative to the controls. Additionally, the treated adult patients had higher plasma levels of Threonine compared to both the active ( p = 0.04) and control ( p = 0.02) subjects, and the increased mRNA expression of IL-4 ( p = 0.01) in comparison to the active patients. In active children with CD, the IL-2 mRNA level was found to be higher than in the controls ( p < 0.0001) and in the treated children ( p = 0.005). The treated children with CD exhibited decreased plasma levels of Tryptophan ( p = 0.01) and Isoleucine ( p = 0.01) relative to the controls, and the increased mRNA expression of TGFβ ( p = 0.04) relative to the active patients. Elevated levels of specific AAs (Serine, Glycine, Threonine) in the treated CD patients suggested their potential to improve intestinal damage and inflammation, while decreased levels of Tryptophan and Isoleucine highlighted the need for dietary intervention.